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It depends on the charger. But for the most part, yes. Most of the modern chargers are trickle chargers and will cut off once the battery is at full capacity. They’ll only turn back on when they need to in order to keep it full. But the benefit of LiFePO4 batteries is that they hold their charge. Once they’re full, you can let them sit and they’ll only lose maybe 1-2%. Sometimes 0%.
